T7 High Efficiency Transcription Kit
T7高效體外轉(zhuǎn)錄試劑盒
| 目錄號 | 規(guī)格 | 單價 |
|---|---|---|
| JT101-01 | 25 rxns | 1160 |
| JT101-02 | 100 rxns | 3700 |
產(chǎn)品詳情介紹
本試劑盒進行了體外轉(zhuǎn)錄體系的優(yōu)化，利用T7 RNA Polymerase，以含有T7啟動子的超螺旋質(zhì)粒DNA或線性DNA為模板，對T7啟動子下游的DNA序列進行高效轉(zhuǎn)錄。本產(chǎn)品適用于制備長度超過6000 nt的高濃度RNA，使用1 μg DNA模板可在20 μl體系生成150~280 μg的RNA (如果想獲得毫克級的RNA產(chǎn)物，可平行放大反應(yīng)體系)。制備的RNA可用于體外翻譯、RNase保護實驗、RNA剪切以及雜交探針標(biāo)記等。
轉(zhuǎn)錄特異性好
轉(zhuǎn)錄產(chǎn)量高
兼容不同類型DNA模板
兼容化學(xué)修飾核苷酸
同類產(chǎn)品對比
熒光鏡檢和流式細(xì)胞分選結(jié)果表明，利用TransGen mRNA體外合成系列產(chǎn)品(JT101)合成的mRNA毒性低、穩(wěn)定性好、翻譯效率高，在不同類型的哺乳動物細(xì)胞中成功且穩(wěn)定表達。
1.Chen Y, Wu Z, Huang S, et al. Adipocyte IRE1α promotes PGC1α mRNA decay and restrains adaptive thermogenesis[J]. Nature metabolism, 2022.(IF 18.90)
2.Zhang Q, Chen Z, Wang F, et al. Efficient DNA interrogation of SpCas9 governed by its electrostatic interaction with DNA beyond the PAM and protospacer[J]. Nucleic Acids Research, 2021.(IF 16.60)
3.Sun H, Li X, Yang X, et al. Low leucine levels in the blood enhance the pathogenicity of neonatal meningitis-causing Escherichia coli[J]. Nature Communications, 2025(15.70)
4.Huang C, Jiang T, Pan W, et al. Ubiquitination of NS1 Confers Differential Adaptation of Zika Virus in Mammalian Hosts and Mosquito Vectors[J]. Advanced Science, 2024. (IF 14.30)
5.Zhang Q, Wen F, Zhang S, et al. The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation[J]. Science advances, 2019.(IF 11.70)
6.Chen K, Sun W, Zhong M, et al. Single-molecule assay guided crRNA optimization enhances specific microRNA detection by CRISPR-Cas12a[J]. Sensors and Actuators B: Chemical, 2024.(IF 8. 00)
7.Hu R, Li M, Chen S, et al. Sniffer restricts arboviral brain infections by regulating ROS levels and protecting blood-brain barrier integrity in Drosophila and mosquitoes[J]. PLoS pathogens, 2024.(IF 5.50)
