TransSerum? EQ Fetal Bovine Serum
胎牛血清 EQ
| 目錄號(hào) | 規(guī)格 | 單價(jià) |
|---|---|---|
| FS201-02 | 500 ml | 詢價(jià) |
產(chǎn)品詳情介紹
本產(chǎn)品以澳洲健康胎牛血液為原料加工而成，經(jīng)3 次0.1 μm 微孔濾膜過(guò)濾，無(wú)支原體污染，不含BVDV、BPV等牛源病毒，內(nèi)毒素水平極低。與FQ FBS 相比，培養(yǎng)細(xì)胞種類更多、細(xì)胞生長(zhǎng)速度更快、狀態(tài)更好，適用于間充質(zhì)干細(xì)胞的培養(yǎng)。培養(yǎng)的細(xì)胞適用于多種下游實(shí)驗(yàn)。
? 澳洲健康胎牛血液為原料加工而成，經(jīng)3 次0.1 μm 微孔濾膜過(guò)濾。
? 無(wú)病毒污染、無(wú)支原體污染、內(nèi)毒素水平低。
? 培養(yǎng)細(xì)胞生長(zhǎng)迅速、倍增時(shí)間短、克隆率高。
? 適用于間充質(zhì)干細(xì)胞等多種類型細(xì)胞的培養(yǎng)。
【高端品質(zhì)型】源自澳洲，廣譜高效且批間差低，是干細(xì)胞、免疫細(xì)胞及原代細(xì)胞等苛刻應(yīng)用的安心之選。
應(yīng)用范圍
適用于間充質(zhì)干細(xì)胞、多能性干細(xì)胞、原代細(xì)胞等多種類型細(xì)胞的培養(yǎng)
TransSerum? EQ Fetal Bovine Serum成功培養(yǎng)的細(xì)胞
病毒檢測(cè)
通過(guò)免疫熒光抗體法檢測(cè)TransGen EQ血清中的病毒殘留，結(jié)果如下：
支原體檢測(cè)
使用全式金支原體檢測(cè)試劑盒 TransDetect? PCR Mycoplasma Detection Kit (FM311)對(duì)Company TH和TransGen的血清進(jìn)行檢測(cè)，結(jié)果表明,Company TH 和TransGen的血清均無(wú)支原體污染。
普通細(xì)胞培養(yǎng)圖 (部分結(jié)果)
配制含有Company TH 和TransGen 10% 血清的生長(zhǎng)培養(yǎng)基，培養(yǎng)不同類型的細(xì)胞，對(duì)其進(jìn)行傳代，在顯微鏡下觀察每一代細(xì)胞的形態(tài)。
特殊細(xì)胞培養(yǎng)圖 (部分結(jié)果)
配制含有 Company TH 和TransGen 10%血清的生長(zhǎng)培養(yǎng)基，培養(yǎng)不同類型的細(xì)胞，在顯微鏡下觀察細(xì)胞的形態(tài)。
TransSerum? EQ Fetal Bovine Serum 培養(yǎng)細(xì)胞的下游實(shí)驗(yàn)
用TransSerum? EQ Fetal Bovine Serum培養(yǎng)的細(xì)胞可以進(jìn)行多種下游實(shí)驗(yàn)，如流式、免疫熒光、免疫共沉淀以及細(xì)胞轉(zhuǎn)染等。下圖展示用TransIntro? EL Transfection Reagent (FT201) 在含血清和不含血清的情況下，對(duì)不同細(xì)胞轉(zhuǎn)染pDsRed-N1質(zhì)粒，培養(yǎng)48 h 后，觀察紅色熒光，結(jié)果表明：血清的有無(wú)對(duì)下游實(shí)驗(yàn)細(xì)胞轉(zhuǎn)染基本沒有影響。
TransSerum? EQ Fetal Bovine Serum成功培養(yǎng)hMSCs
含TransGen血清的生長(zhǎng)培養(yǎng)基培養(yǎng)hMSCs至第5代細(xì)胞形態(tài)圖及培養(yǎng)不同代數(shù)細(xì)胞倍增數(shù)圖，結(jié)果表明，TransGen血清可成功培養(yǎng)hMSCs至第10代。
流式細(xì)胞術(shù)檢測(cè)培養(yǎng)至第8代的hMSCs 表面陽(yáng)性基因表達(dá)高于97%，陰性標(biāo)記基因表達(dá)低于0.06%，結(jié)果表明，TransGen血清培養(yǎng)的hMSCs純度高。
油紅O染色、茜素紅染色、阿爾新藍(lán)-核固紅染色分別檢測(cè)hMSCs誘導(dǎo)分化為脂肪細(xì)胞、成骨細(xì)胞和軟骨細(xì)胞。
結(jié)果表明，TransGen 血清培養(yǎng)的hMSCs 可成功誘導(dǎo)分化為脂肪細(xì)胞、成骨細(xì)胞和軟骨細(xì)胞。
1.Liu Q, Cheng H B, Ma R, et al. A DNA-based nanodevice for near-infrared light-controlled drug release and bioimaging[J]. Nano Today, 2023.(IF 17.40)
2.Li Z, Wang D, Lu J, et al. Methylation of EZH2 by PRMT1 regulates its stability and promotes breast cancer metastasis[J]. Cell Death & Differentiation, 2020.(IF 10.71)
3.Guo C, Gao C, Zhao D, et al. A novel ETV6-miR-429-CRKL regulatory circuitry contributes to aggressiveness of hepatocellular carcinoma[J]. Journal of Experimental & Clinical Cancer Research, 2020.(IF 7.06)
4.Zhang Q, Li R L, Tao T, et al. Antiepileptic effects of Cicadae periostracum on mice and its antiapoptotic effects in H2O2‐stimulated PC12 cells via regulation of PI3K/Akt/Nrf2 signaling pathways[J]. Oxidative Medicine and Cellular Longevity, 2021.(IF 6.54)
5.Sun X, Tao B, Wang Y, et al. Isolation and characterization of germline stem cells in protogynous hermaphroditic Monopterus albus[J]. International Journal of Molecular Sciences, 2022.(IF 6.21)
6.Guo P, Xu J, Liang H, et al. Estrogen suppresses cytokines release in cc4821 neisseria meningitidis infection via TLR4 and ERβ-p38-MAPK pathway[J]. Frontiers in microbiology, 2022.(IF 6.06)
7.Wang X, Hu M, Chen J, et al. Key roles of autophagosome/endosome maturation mediated by Syntaxin17 in methamphetamine-induced neuronal damage in mice[J]. Molecular Medicine, 2024,(IF 6.00)
8.Liu S, Liang H, Lv L, et al. 3D culture boosting fullerenol nanoparticles to induce calreticulin exposure on MCF-7 cells for enhanced macrophage-mediated cell removal[J]. Colloids and Surfaces B: Biointerfaces, 2023.(IF 5.99)
9.Zhang Q, Qian D, Tang D D, et al. Glabridin from Glycyrrhiza glabra possesses a therapeutic role against keloid via attenuating pi3k/akt and transforming growth factor-β1/smad signaling pathways[J]. Journal of Agricultural and Food Chemistry, 2022.(IF 5.89)
10.Sun J Y, Liu Y T, Jiang S N, et al. Essential oil from the roots of Paeonia lactiflora pall. has protective effect against corticosterone-induced depression in mice via modulation of PI3K/Akt signaling pathway[J]. Frontiers in Pharmacology, 2022.(IF 5.98)
11.Zhou M, Tao X, Lin K, et al. Downregulation of the HCN1 Channel Alleviates Anxiety‐and Depression‐Like Behaviors in Mice With Cerebral Ischemia–Reperfusion Injury by Suppressing the NLRP3 Inflammasome[J]. Journal of the American Heart Association, 2025.(IF 5.30)
