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TransScript? One-Step gDNA Removal and cDNA Synthesis SuperMix

TransScript一步法去除gDNA及第一鏈cDNA合成試劑盒

目錄號規(guī)格單價
AT311-0250 rxns×20 µl體系1160
AT311-03100 rxns×20 µl體系2080
AT311-04 500 rxns×20 µl體系9350
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產(chǎn)品詳情介紹

本產(chǎn)品以RNA為模板,在同一反應(yīng)體系中,合成第一鏈cDNA的同時去除RNA模板中殘留的基因組DNA。反應(yīng)結(jié)束后,只需在85℃加熱2分鐘,即可同時失活TranScript? RT/RI與gDNA Remover。

產(chǎn)品組成

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使用前請將各組分點(diǎn)甩離心

實(shí)驗(yàn)數(shù)據(jù)

PCR 檢測

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qPCR 檢測

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References

1.Gong Q, Wang Y, He L, et al. Molecular basis of methyl-salicylate-mediated plant airborne defence[J]. Nature, 2023.(IF 64.80)

2.Guan J, Wang G, Wang J, et al. Chemical reprogramming of human somatic cells to pluripotent stem cells[J]. Nature, 2022.(IF 49.00)

3.Huang Y Y, Sun Y M, Qi H Y, et al. A conserved H3K14ub-driven H3K9me3 for chromatin compartmentalization [J]. Nature, 2025.(IF 48.50)

4.Wang J Z, Chen T Y, Zhang Z D, et al. Remodelling autoactive NLRs for broad-spectrum immunity in plants [J]. Nature, 2025.(IF 48.50)

5.Wang S, Du Y, Zhang B, et al. Transplantation of chemically induced 5.pluripotent stem-cell-derived islets under abdominal anterior rectus sheath in a type 1 diabetes patient[J]. Cell,  2024.(IF 45.50)

6.Zhao P Z, Yang H, Sun J Y, et al. Targeted MYC2 stabilization confers citrus Huanglongbing resistance [J]. Science, 2025.(IF 44.70)

7.Chen J, Ou Y, Luo R, et al. SAR1B senses leucine levels to regulate mTORC1 signalling[J]. Nature, 2021.(IF 42.77)

8.Ma X J, Wang W, Zhang J Y, et al. NRT1.1B acts as an abscisic acid receptor in integrating compound environmental cues for plants[J]. Cell, 2025.(IF 42.50)

9.Chen J, Ou Y, Yang Y, et al. KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing[J]. Nature, 2018.(IF 40.13)

10.Zhao K, Xue H, Li G, et al. Pangenome analysis reveals structural variation associated with seed size and weight traits in peanut[J]. Nature genetics, 2025.(IF 31.80)

11.Liu S, Liu C, Lv X, et al. The chemokine CCL1 triggers an AMFR-SPRY1 pathway that promotes differentiation of lung fibroblasts into myofibroblasts and drives pulmonary fibrosis[J]. Immunity, 2021.(IF 31.74)

12.Huang L, Wei M, Li H, et al. GP73-dependent regulation of exosome biogenesis promotes colorectal cancer liver metastasis[J]. Molecular Cancer, 2025.(IF 27.70)

13.Fan H, Quan S, Ye Q, et al. A molecular framework underlying low-nitrogen-induced early leaf senescence in Arabidopsis thaliana[J]. Molecular Plant, 2023.(IF 27.50)

14.Zhu J, Zhong X, He H, et al. Generation of human expandable limb-bud-like progenitors via chemically induced dedifferentiation[J]. Cell Stem Cell, 2024.(IF 19.80)

15.Wang R, Xue Y, Fan J, et al. A systems genetics approach reveals PbrNSC as a regulator of lignin and cellulose biosynthesis in stone cells of pear fruit[J]. Genome Biology, 2021.(IF 17.90)

16.Zhang Y, Chen W, Wu D, et al. Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli[J]. Nucleic acids research, 2022.(IF 16.97)

17.Wang Z, Sheng C, Kan G, et al. RNAi Screening Identifies that TEX10 Promotes the Proliferation of Colorectal Cancer Cells by Increasing NF‐κB Activation[J]. Advanced Science, 2020.(IF 15.84)

18.Zhao M, Wang B, Zhang C, et al. The DJ1-Nrf2-STING axis mediates the neuroprotective effects of Withaferin A in Parkinson’s disease[J]. Cell Death & Differentiation, 2021.(IF 15.82)

19.Liu J C, Li L, Yan H C, et al. Identification of oxidative stress–related Xdh gene as a di (2‐ethylhexyl) phthalate (DEHP) target and the use of melatonin to alleviate the DEHP‐induced impairments in newborn mouse ovaries[J]. Journal of pineal research, 2019.(IF 15.22)

20.Zhang C, Wang Y, Zhen Z, et al. mTORC1 Mediates Biphasic Mechano‐Response to Orchestrate Adhesion‐Dependent Cell Growth and Anoikis Resistance[J]. Advanced Science, 2023.(IF 15.10)

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