TransSerum? EQ Fetal Bovine Serum
胎牛血清 EQ
目錄號(hào)： FS201-02
單 價(jià)：￥詢價(jià)
產(chǎn)品詳情介紹
本產(chǎn)品以澳洲健康胎牛血液為原料加工而成，經(jīng)3 次0.1 μm 微孔濾膜過濾，無支原體污染，不含BVDV、BPV等牛源病毒，內(nèi)毒素水平極低。與FQ FBS 相比，培養(yǎng)細(xì)胞種類更多、細(xì)胞生長速度更快、狀態(tài)更好，適用于間充質(zhì)干細(xì)胞的培養(yǎng)。培養(yǎng)的細(xì)胞適用于多種下游實(shí)驗(yàn)。
? 澳洲健康胎牛血液為原料加工而成，經(jīng)3 次0.1 μm 微孔濾膜過濾。
? 無病毒污染、無支原體污染、內(nèi)毒素水平低。
? 培養(yǎng)細(xì)胞生長迅速、倍增時(shí)間短、克隆率高。
? 適用于間充質(zhì)干細(xì)胞等多種類型細(xì)胞的培養(yǎng)。
【高端品質(zhì)型】源自澳洲，廣譜高效且批間差低，是干細(xì)胞、免疫細(xì)胞及原代細(xì)胞等苛刻應(yīng)用的安心之選。
應(yīng)用范圍
適用于間充質(zhì)干細(xì)胞、多能性干細(xì)胞、原代細(xì)胞等多種類型細(xì)胞的培養(yǎng)
TransSerum? EQ Fetal Bovine Serum成功培養(yǎng)的細(xì)胞
病毒檢測(cè)
通過免疫熒光抗體法檢測(cè)TransGen EQ血清中的病毒殘留，結(jié)果如下：
支原體檢測(cè)
使用全式金支原體檢測(cè)試劑盒 TransDetect? PCR Mycoplasma Detection Kit (FM311)對(duì)Company TH和TransGen的血清進(jìn)行檢測(cè)，結(jié)果表明,Company TH 和TransGen的血清均無支原體污染。
普通細(xì)胞培養(yǎng)圖 (部分結(jié)果)
配制含有Company TH 和TransGen 10% 血清的生長培養(yǎng)基，培養(yǎng)不同類型的細(xì)胞，對(duì)其進(jìn)行傳代，在顯微鏡下觀察每一代細(xì)胞的形態(tài)。
特殊細(xì)胞培養(yǎng)圖 (部分結(jié)果)
配制含有 Company TH 和TransGen 10%血清的生長培養(yǎng)基，培養(yǎng)不同類型的細(xì)胞，在顯微鏡下觀察細(xì)胞的形態(tài)。
TransSerum? EQ Fetal Bovine Serum 培養(yǎng)細(xì)胞的下游實(shí)驗(yàn)
用TransSerum? EQ Fetal Bovine Serum培養(yǎng)的細(xì)胞可以進(jìn)行多種下游實(shí)驗(yàn)，如流式、免疫熒光、免疫共沉淀以及細(xì)胞轉(zhuǎn)染等。下圖展示用TransIntro? EL Transfection Reagent (FT201) 在含血清和不含血清的情況下，對(duì)不同細(xì)胞轉(zhuǎn)染pDsRed-N1質(zhì)粒，培養(yǎng)48 h 后，觀察紅色熒光，結(jié)果表明：血清的有無對(duì)下游實(shí)驗(yàn)細(xì)胞轉(zhuǎn)染基本沒有影響。
TransSerum? EQ Fetal Bovine Serum成功培養(yǎng)hMSCs
含TransGen血清的生長培養(yǎng)基培養(yǎng)hMSCs至第5代細(xì)胞形態(tài)圖及培養(yǎng)不同代數(shù)細(xì)胞倍增數(shù)圖，結(jié)果表明，TransGen血清可成功培養(yǎng)hMSCs至第10代。
流式細(xì)胞術(shù)檢測(cè)培養(yǎng)至第8代的hMSCs 表面陽性基因表達(dá)高于97%，陰性標(biāo)記基因表達(dá)低于0.06%，結(jié)果表明，TransGen血清培養(yǎng)的hMSCs純度高。
油紅O染色、茜素紅染色、阿爾新藍(lán)-核固紅染色分別檢測(cè)hMSCs誘導(dǎo)分化為脂肪細(xì)胞、成骨細(xì)胞和軟骨細(xì)胞。
結(jié)果表明，TransGen 血清培養(yǎng)的hMSCs 可成功誘導(dǎo)分化為脂肪細(xì)胞、成骨細(xì)胞和軟骨細(xì)胞。
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